zen software version 2012  (Carl Zeiss)

Journal: International Journal of Molecular Sciences

Article Title: Exposure of Colon-Derived Epithelial Monolayers to Fecal Luminal Factors from Patients with Colon Cancer and Ulcerative Colitis Results in Distinct Gene Expression Patterns

doi: 10.3390/ijms25189886

Figure Lengend Snippet: Fecal metabolite profiles and characterization of the RepliGut ® Planar monolayers treated with fecal supernatants (FS). ( A ) A principal component analysis based on 9699 spectral features detected in FS, analyzed by untargeted liquid chromatography/mass spectrometry, for healthy subjects (HS, n = 6, green dots), patients with ulcerative colitis (UC, n = 6, red dots) and colon cancer patients (CC, n = 6, blue dots). ( B ) Primary human intestinal cells in the RepliGut ® Planar system form a polarized monolayer with an apical membrane morphologically comparable with that of human intestine, as visualized by apical localization of phospho-ezrin (green), and actin filaments as detected by fluorescent phalloidin (magenta). Nuclei are visualized in blue color. The right hand and bottom panels show the orthographic view of the region, where “XY” and “XZ” indicate different cross-sections. The image was acquired with LSM700 inverted confocal microscope; 63× magnification. ( C ) Transepithelial electrical resistance was measured before and at 24 h and 48 h after addition of FS (HS: green dots, UC: red dots, CC: blue dots), TNFα (black triangles) or untreated (Media, gray triangles).

Article Snippet: Images of stained monolayers were acquired using 20× and/or 40× objective and the same acquisition settings on an LSM 700 inverted confocal microscope (Carl Zeiss, Oberkochen, Germany) using Zen 2012 SP5 (Black edition, version 14.0.3.201) imaging software.

Techniques: Liquid Chromatography, Mass Spectrometry, Membrane, Microscopy